Coding
Part:BBa_K2381000:Design
Designed by: Wenqi Huang Group: iGEM17_HZAU-China (2017-10-20)
pMagFast
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 87
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The protein sequence is acquired from Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256, and its DNA sequence is codon optimized for E. coli by IDT.
Source
This part is made from the VVD protein family derived from the fungus Neurospora crassa.
References
Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256